Tris(hydroxymethyl)aminomethane hydrochloride, CAS No. 1185-53-1

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1185-53-1

Catalog No. EBD22692

CAS 1185-53-1

Name Tris(hydroxymethyl)aminomethane hydrochloride

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Basic Information

Synonyms: Tris(hydroxymethyl)aminoethanehydrochloride Tris(hydroxymethyl)aminomethanehydrochloride 2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL,HYDROCHLORIDE SPECTRIS(R)HYDROCHLORIDE SPECTRIS(TM)HYDROCHLORIDE MOLE-READY-TRIS 1,3-Propanediol,2-amino-2-(hydroxymethyl)-,hydrochloride 3-propanediol,2-amino-2-(hydroxymethyl)-hydrochloride trizmahydrochloride(trishydrochloride) 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride(1:1) 1,3-propanediol,2-amino-2-(hydroxymethyl)-,chloride TRIS·HCL TRIZMAHydrochloride TrisHydrochloride 2-Amino-2-(hydroxymethyl)-1,3-propanediolhydrochloride Tris.HCl TRIS-HCL tri(hydroxymethyl)amino

Molecular Formula: C4H11ClNO3

Molecular Weight: 157.6

MDL Number: MFCD00012590

Categories: Medicinal Chemistry > Pharmaceutical Excipients > Isotonic Agents, pH Adjusters, and Buffers

Product Description:

Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), with CAS number 1185-53-1, is the hydrochloride salt of Tris base, a primary amine with the formula (HOCH_)_CNH_бдHCl. It is a white crystalline powder that is highly soluble in water, forming a clear solution. Its structure features three hydroxyl groups and a protonated amine, making it a zwitterionic compound at physiological pH. Tris-HCl is primarily used as a buffering agent in biochemical and molecular biology applications, maintaining a stable pH range of 7.0 to 9.0. It is a standard component in electrophoresis buffers (e.g., TAE, TBE), protein purification systems, and enzymatic assays. Its low ionic strength and minimal interference with biological reactions make it ideal for DNA/RNA work and cell culture media. As a general laboratory reagent, Tris-HCl also serves as a pH standard and a starting material for synthesizing other Tris derivatives. It is non-toxic, stable under normal conditions, and widely available in research-grade purity. Its versatility in both life sciences and synthetic chemistry underscores its dual classification.

Physical Properties

Melting Point: 150-152 °C

Boiling Point: 357 ºC at 760 mmHg

Flash Point: 169.7 ºC

Density: 1.05 g/mL at 20 °C

Solubility: H2O: 4 M at 20 °C, clear, colorless

Storage: Store at RT.

Analytical Data

Appearance: White crystalline powder